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Yes, overspraying can lead to bacterial/mold buildup and cause your cham to become sick. You also want the enclosure to completely dry in between misting and before the lights go out.
Can you over spray a new chameleon. I usually wait about a hour when the lights come on and wont spray 1 hour before lights out but in between I spray once ever 1-2 hours is this too much.
How often you need to spray depends a lot on the humidity level you are trying to maintain in the cage and how dry the room tends to be. If there is a LOT of live foliage in the cage to catch the droplets you probably don't need to spray as often. We can't tell you exactly how much to spray because everyone's situation is different.
Questions:
How do you measure cage humidity now? What is the range from dry to wet?
Do you use live plants in the cage?
What are the temps in the room now?
Is your cham always thirsty and drinking every time you spray?
You can get more good information about the importance of spraying, humidity, etc. if you read the cham care section under the forum Resources tab. Also tips and suggestions for doing it correctly.
To answer your questions:
Only had him for two days now he usually hides when I spray then when I go across room sometimes he drinks others he doesn't.
I have one live plant in his cage it is about 1/4-1/3 size of cage
the temps in the room are around 65 I live in a old house have a digital reading near basking spot its around 90-95 and a analog on bottom its around 70 I measuring humidity I have questions with. I have a digital thermometer/hygrometer where should I put the hyg lead if I place it on side it reads lower than if it were in middle
Yes, overspraying can lead to bacterial/mold buildup and cause your cham to become sick. You also want the enclosure to completely dry in between misting and before the lights go out.
Actually, I don't think you would want the ENTIRE cage to dry completely. The areas that would normally be more humid (in denser parts of the foliage for example) shouldn't get bone dry. What you want to try to do is provide a range of different humidity levels...the driest and warmest under the lights where your cham basks, but more humid areas that don't sit completely stagnant with no air exchange. How fast the cage dries out will also depend on how much air exchange there is in the room, how you heat your house, your local climate, and the room's humidity. More air flow, faster drying and less chance of mold. It takes time, trial and error to fine tune a particular cage setup.