Definitely. In general, a chameleon should be afriad of you, especially when they first arrive and doubly so when you are dealing with a wild caught. A deviation from that natural fear suggests illness and weakness. Everything you have written in this post today suggests he is doing poorly and should see a vet.
Either the leg is getting worse or you are now only noticing that he feels pain with it. An animal has a very strong survival instinct, so will use very painful limbs.
I think you said that you had an exotic vet lined up? It is probably a good idea to take him/them in. If he were mine, based on your description today, I would have him in to the vet today.
If he is a wild caught, recent or otherwise, I am quite sure he has parasites, and lots of them. It is not wise to try to deparasitize a chameleon in poor health. They tend to die. I could give you my vet's explanation of why it is extremely risky to try to worm wild caughts and how it needs to be done very carefully when the animal is in good health. It has nothing to do with the safety/toxicity of the worming meds and all to do with what happens when you have a mass die off of parasites not to mention the very serious negative effect the stress of handling to worm will have on the animal.
By the way, I have one wild caught that my vet and I refer to as He Who Will Not Die. He has been on death's doorstep countless times. I've had him in to the vet when he was so sick, he was limp in her hands--that's one very sick chameleon. I've made appointments to euthanize and do the necropsy only to cancel and try one last treatment for something my vet was sure he didn't have because he definitely was not presenting that way, but why not, we could always euthanize later. We have no idea what is troubling him, but he continues to live and eat and grow and actually is doing very well right now. We suspect he has either lung worms or an abscess somewhere in his body or both. Possibly a tumor. My vet and I are both looking forward to doing the necropsy when he finally does die, which we both hope if from old age. He's been an interesting riddle and we are both amazed he is alive at all and actually doing well. He is one tough lizard and we both are in awe with his immune system because he really should be dead. He's a special chameleon, I just wish I could get him to breed. I sure would like his resilience in the gene pool.
. Anytime i had to handle my animals more than usual i blacked the enclosures out, i just taped black plastic on all side panels to reduce stress. Always worked well for me.
