Continued debate on Vit A...

Zach Valois

New Member
Well... I am posting to bring to light a new product called Sunshine Factor.

I have been working with good exotics vet and she is my acting consultant.

The first topic is that, despite a few posts I have seen about it, vit a levels cannot be accurately determined via blood tests (per comm from vet). Vitamin A is a fat stored vitamin and thus can only by done by a liver necropsy or assay of the liver. So that is one note..

Furthermore, I am increasingly skeptic on how often us culturists jump to a diagnosis of hypovitaminosis A. I have not seen a single case (please share if you have) of a necropsied specimen, yielding firm results of deficient Vit A levels (or hypervitaminosis for that matter). Now I am not disputing that this is a problem, because numerous published sources have discussed the common nature of this topic in other herps (see Mader 2006).

As far as beta carotene is concerned, I think we have established that it is not known if reptiles can use it in this form. Well, per the information supplied by my vet, herbivorous reptiles should be able to use beta carotene. However, carnivorous [reptiles] animals cannot. Going on, insectivorous herps chemically could be considered omnivorous (further supported by vegetation consumption as we see in some chameleon taxa). With that said, in theory, insectivorous herps should be able to convert beta carotene. However, it is undefined as to how complete this form converts over and if and what components are being missed.

Ferguson et al 2004, provided some input on the matter.
"Panther chameleons fed crickets gut loaded with standard Zeigler diet (70iu/g of Vit A) have rarely shown signs of Vit A deficiency. Half grown crickets on this standard diet contain roughly 15% of the concentration of the diet or about 10 iu/g of vit A (Ferguson et al. 1996). If Vit A deficiency appears (for example, when old diet contained degraded Vit A is used), it can be corrected with a single dose of 4,000 to 8,000 iu of Vit A suspended in corn oil administered orally. We recommend a chronic daily dose of 5-10 iu of Vit A, obtained by gut loaded crickets or mealworms a diet containeing roughly seven times that concentration. A weekly oral dose of 30-40 IU for an adult chameleon fed crickets gut loaded with low Vit A diets has also been reported to be sufficient. A diet containing caretonoids but not Vit A does not seem to be effective at preventing Vit A deficiency symptoms in panther chameleons"

Ferguson et al 2006 goes on to outline the importance of feeding a high calcium dry food mix in addition to mixed vegetables, with some interesting notes on nutrient comparison of smaller crickets as oppose to adult crickets.

From their 2006 publication, "Accordingly, we tend to feed larger numbers of smaller crickets (half-grown size or smaller) and mealworms (medium sized or smaller) to even the largest male panther chameleons."

I must note however, that signs of Vit A deficiency as described by the authors is not clearly outlined, at least not in this publication.

Some interesting food for thought here.

And finally, I wanted to see if anyone has heard of a new product called Sunshine Factor. It is primarily Red Palm fruit oil. It reads to have high carotene content, this being a precursor of Vit A. With such being a precursor, there is a smaller inherent risk of vitamin toxicity. But, can and will sufficient Vit A be derived from the product?

I have a trio of animals that all came from one breeder and they are markedly smaller and stunted compared to my other animals. Their appetite is a 1/3 of what my other animals eat and obviously have something going on. I have given an intial dose of 0.1 ml of Sunshine Factor and will continue to gut load some staple prey items with it and see if I see any changes.

The product is being marketed primarily for birds and reptiles, but is said to be applicable to virtually any animal. Here is a link:

http://www.harrisonsbirdfoods.com/SUNSHINE-FACTOR-1oz/productinfo/AVXSSF1/



In conclusion, I think I will modify my gutloading to always include a high calcium dry food (I may or may not put Sunshine Factor in the food), in addition to the usual assortment of collards, sweet potatoes, carrots, etc. Ferguson et al. 2006 did mention that smaller mealworms and to a lesser degree, crickets, transfer calcium more effectively when gut loaded.

Ferguson does have a few more publications that I am going to try and track down.. all pertaining to the issue at hand, and I am surprised that no one has strongly referenced them (that I have come across that is).



Your thoughts?
 
One question I still have is why would there be beta carotene in the eggs of panther chameleons if they can't convert it? What purpose would it be serving?

"b. carotene concentrations were of the same magnitude as grazing tortoises."...
czaw.net/node/3492

Also...the only case of hypovitaminosis A that was ever reported in one of my chameleons (and I have had many many necropsied) was in a chameleon that had crickets that were dusted with vitamins that contained prEformed vitamin A.
 
One question I still have is why would there be beta carotene in the eggs of panther chameleons if they can't convert it? What purpose would it be serving?

"b. carotene concentrations were of the same magnitude as grazing tortoises."...
czaw.net/node/3492

Also...the only case of hypovitaminosis A that was ever reported in one of my chameleons (and I have had many many necropsied) was in a chameleon that had crickets that were dusted with vitamins that contained prEformed vitamin A.


So this is delving into something I have virtually no research on. That is more reproductive physiological stuff (would that be ontogenic, embyrologic, or what?).

Where is that quote from? Are you saying that the egg shells themselves contain b carotene, the amniotic fluid, sac?

So, you are reporting that you had a confirmed case of an animal that was in fact Vit a deficient? How did that relate to the decline and eventually diagnosed cause of death with the animal?

Also, if and what did you change since the issues surrounding that animal? I assume you were using repcal multivit?
 
It's to do with the egg yolk in the eggs of panther chameleons.

I gave the Site address of where I took the quote from....
Czaw.net.net/node/3492

There was no real diagnosis of what was wrong with the animal that led to its death. All I can tell you is that it was one of a clutch that came from WC parents that were bred in captivity. The rest of the clutchbdid well but at 6 months of age this one was no bigger than a 2 month old...looked healthy...acted normal...just never grew much. All were cared for exactly the same. It was a chameleon chamaeleon female.

The vitamin powder I used had prEformed vitamin A in it asi mentioned...so there should not have been any vitamin A shortage like there might have been if the vitamin powder contained prOformed vitamin A. It was Necton Rep.

Any changes I made later to the supplementing, etc had nothing to do with this chameleon.
 
I thought eggs consist of both pro and preformed Vit A?

I am interested to see your results with sunshine factor. Keep us posted.

As far as vitA is concerned with my experiences with panthers specifically. I can tell you for reasons i do not know specifically but do wonder if the organs are functioning properly.. Some can and some cant convert proformed vitA sources i have offered. The "hypo" situations encountered often had a result of one eye closed, sometimes accompanied by the lack of movement and appetite. These particular chameleons were treated with a preformed source of vit A to resolve the issue. This is now the reason i implement a preformed source periodically in my panthers diet.

Not every species of chameleon has the same dietary needs. ;)
 
It's to do with the egg yolk in the eggs of panther chameleons.

I gave the Site address of where I took the quote from....
Czaw.net.net/node/3492

There was no real diagnosis of what was wrong with the animal that led to its death. All I can tell you is that it was one of a clutch that came from WC parents that were bred in captivity. The rest of the clutchbdid well but at 6 months of age this one was no bigger than a 2 month old...looked healthy...acted normal...just never grew much. All were cared for exactly the same. It was a chameleon chamaeleon female.

The vitamin powder I used had prEformed vitamin A in it asi mentioned...so there should not have been any vitamin A shortage like there might have been if the vitamin powder contained prOformed vitamin A. It was Necton Rep.

Any changes I made later to the supplementing, etc had nothing to do with this chameleon.


Keep in mind that fetal physiology can be quite different from that of a nonfetus. A primary example of this is how fetal hemoglobin is quite different than normal hemoglobin. Just throwing that out there for what it's worth.
I spent a number of years of my life specifically researching these differences in extremely premature infants, and how these physiological shifts changed required care.
 
Yes, certainly, I would agree that properly gutloading increases the nutrients being passed along to the chameleon.

As for the tired (to me) debate about vitamin A....
Probably too many variables (each species could have different abilities/needs. For example, We often read about the needs on montane animals being less than veileds; different prey insects may provide different nutrients; gutloading varies from keeper to keeper; natural sunlight exposure or UVB tubes only (D matters to A), etc.

There are lots of threads already on this topic, if you are interested. These are some of them:

https://www.chameleonforums.com/food-thought-12472
http://web.archive.org/web/200605020...Vitamin.A.html
https://www.chameleonforums.com/chame...nt-want-21534/
Seriously worried.
https://www.chameleonforums.com/carrots-vit-23572
http://jgp.rupress.org/cgi/reprint/47/3/433.pdf
Vitamin A....it scares me
http://www.peteducation.com/article....+1796&aid=3027
Insects and vitamin A and other nutrients.
Vitamins and othr nutrients...
http://www.mendeley.com/research/car...ifer-pardalis/
Vitamin A Supplements From Dr. Ivan Alfonso's Blog
Supplement Confusion
http://www.uvma.org/chameleon/vitaminA.htm
https://www.chameleonforums.com/gutload-vitamin-27997/
https://www.chameleonforums.com/blogs/sandrachameleon/65-supplements.html

I personally raised healthy, breeding, long-lived panthers without using any pre-formed vitamin A supplement. Herptivite was my vitamin supplement (carotene) and even that wasnt used all that often.

My experience does NOT match what appears to be a more common experience on this forum, but my experience is also not unique.

More recently, since I was given a small bag of repashy calcium plus and since my herptivite has expired, I have been using repashy calicum plus (contains vitamin A) about 3 times a week (significantly less than what the instructions recommend, but all that I felt was warranted). It hasnt done any harm, nor has it offered any bonus - as far as I can notice.
There are many "all in one" type products that contain vitamin A and which seem to be useful, used in moderation.
 
Like you sandrachameleon...I have not given my panther chameleons prEformed vitamin A and they have done well. I wonder if, when a panther chameleon develops a symptoms of a lack of vitamin A it might have something to do with how much D3 the chameleon is getting?
 
More recently, since I was given a small bag of repashy calcium plus and since my herptivite has expired, I have been using repashy calicum plus (contains vitamin A) about 3 times a week (significantly less than what the instructions recommend, but all that I felt was warranted).


oops that should say three times a month.
 
Like you sandrachameleon...I have not given my panther chameleons prEformed vitamin A and they have done well. I wonder if, when a panther chameleon develops a symptoms of a lack of vitamin A it might have something to do with how much D3 the chameleon is getting?

Interesting note. I will have more to add on this later.. after some select discussion with a few individuals.

This brings up my main concern. Exactly what are the symptoms of Vit A deficiency and how often are the symptoms really a sign of such a deficiency?

I have seen little, if hardly any clinical results connecting observed symptoms with actual Vit A def.

Not debating this to any degree, I would just like to see some clinical quantitative results. I will be working with a vet as an employee in the near future on this topic and we plan to delve further into this.

Thanks for the input,

Zach
 
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