Most answers will most likely be no, it is best to have fresh gut loaded feeders everyday. If you are not going to be around for a day, it can work to have a few feeders in a cup such as super worms or another bug that can't jump out of the cup and hide in the chameleons cage and bother them over night. Make sure to punch small holes in the cup so when you mist the cup doesn't fill with water and drown the feeders. Good luck hope this was of some help