Blood smear/ WC F. pardalis/ parasites..

Looks like the preview had most of the information on it. It's encouraging to see work like this being actively done. It appears that is was not essentially chameleon experts doing this project, but the interest and motivation was still there. This kind of stuff makes me remember why I should be pushing my career choices out of the pet industry!

I am curious as to what they have listed in their work cited page.

Thanks again for sharing!
 
Howdy Lynda,

I remember that article :). I think it was that article triggered me to look at necropsy blood samples along with fecal testing. I was shocked to see just how many of those Microfilariae parasites could be found in a drop of blood from a WC panther (it was a friend's panther) that had been alive just hours before :eek:. It almost seemed like there was a worm for every 100 blood cells. Here's a few videos that I took of those samples:

http://www.youtube.com/watch?v=xpvOfNZ4lJI

http://www.youtube.com/watch?v=Vdd0NWqTtNo

http://www.youtube.com/watch?v=u_jzQ9ND6gY

http://www.youtube.com/watch?v=pm5ySS3rrvE
 
WOW:eek:
How would you treat some thing like that?:eek:

Dave Weldon said:
Howdy Lynda,

I remember that article :). I think it was that article triggered me to look at necropsy blood samples along with fecal testing. I was shocked to see just how many of those Microfilariae parasites could be found in a drop of blood from a WC panther (it was a friend's panther) that had been alive just hours before :eek:. It almost seemed like there was a worm for every 100 blood cells. Here's a few videos that I took of those samples:

http://www.youtube.com/watch?v=xpvOfNZ4lJI

http://www.youtube.com/watch?v=Vdd0NWqTtNo

http://www.youtube.com/watch?v=u_jzQ9ND6gY

http://www.youtube.com/watch?v=pm5ySS3rrvE
Click to expand...
 
I actually work at a medical research company and I am a microscopy technician, specializing in live blood analysis through the microscope :)

I can say parasites are fairly common in live blood.. (you should have seen mine after getting back from Nigeria) although you rarely if ever see any that large. For example some staff infections look like a dragon swimming by throwing all the RBC's out of the way..and I even think it can breath fire. That parasite in that picture is much larger than your common parasites :) although...i havnt looked at chameleon blood yet...I just couldnt stand to prick one of those little guys.


But if anyone wants to take a look at their cham's blood in Orange County, we can do that and make a little video out of it or something /shrug
-.-;
 
Jordan123 said:
I actually work at a medical research company and I am a microscopy technician, specializing in live blood analysis through the microscope :)...
Click to expand...
Howdy Jordan,

You have a standing invitation to tell us about microscopy at one of our South Bay Chameleon Keeper meetings :D! We've been hoping to find someone in that area of expertise for quite a while. No pressure - just chat with the group about anything relevant to using a microscope and chasing parasites :eek:. There are 3-4+ of us with 'scopes that would like to know more and the rest of the group would benefit from the added knowledge too. The next mtg will likely be towards the end of July.
 
how did you film this?

Hi

I am a DVM in Virginia. I'm curious as to what equipment you used to make your movies?

Incidentally I had a similar case today. A positive parasite ID is pending.

Dr. Silverstone
 
Thats amazing! Shakes head at the unknown battles you can endure with w/c.

I dont know if this was in the article. So, please excuse my lack of retaining most of what i read. any idea how it was contracted?
 
igzoticdvm said:
Hi

I am a DVM in Virginia. I'm curious as to what equipment you used to make your movies?

Incidentally I had a similar case today. A positive parasite ID is pending.

Dr. Silverstone
Click to expand...
Howdy Doc,

Here's a post of mine from a few years ago that lists the low cost (currently $149 w/free shipping) 1.3MP video camera that slips into either the trinocular tube or just pull out an eyepieces fro a binocular 'scop[e or just replace the eyepiece in a monocular 'scope. They work pretty well. You just plug it into a PC USB port and let the software capture either video or snapshots.

https://www.chameleonforums.com/microscope-preferences-26015/#post241982

Here's a direct link to one of little video cameras available on eBay today: http://cgi.ebay.com/USB-1-3-Mega-Pi...958?pt=LH_DefaultDomain_0&hash=item3caa0ebf4e
 
Dave Weldon said:
Howdy Jordan,

You have a standing invitation to tell us about microscopy at one of our South Bay Chameleon Keeper meetings :D! We've been hoping to find someone in that area of expertise for quite a while. No pressure - just chat with the group about anything relevant to using a microscope and chasing parasites :eek:. There are 3-4+ of us with 'scopes that would like to know more and the rest of the group would benefit from the added knowledge too. The next mtg will likely be towards the end of July.
Click to expand...

I absolutely second this!! Iv got the microscopy 'basics' pretty well down. But I tend to pick apart plants and everything else (that contains no blood) but i am looking to expand :):).

To everyone else this is an awesome thread.
 
Dave Weldon said:
Howdy Lynda,

I remember that article :). I think it was that article triggered me to look at necropsy blood samples along with fecal testing. I was shocked to see just how many of those Microfilariae parasites could be found in a drop of blood from a WC panther (it was a friend's panther) that had been alive just hours before :eek:. It almost seemed like there was a worm for every 100 blood cells. Here's a few videos that I took of those samples:

http://www.youtube.com/watch?v=xpvOfNZ4lJI

http://www.youtube.com/watch?v=Vdd0NWqTtNo

http://www.youtube.com/watch?v=u_jzQ9ND6gY

http://www.youtube.com/watch?v=pm5ySS3rrvE
Click to expand...

wow. It looks like 1-100 or worse! That is so gnarly, alive hours before those slides were made.
 
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