Howdy Dan,

We should probably get a "back-fill" of history info of the situation along with the usual: How to ask for help info . Depending on the history, I wouldn't rule out eye infections or even general stress for the moment. It will be useful to know the overall room humidity. Chameleons that are "good" drinkers might do ok with short misting sessions while picky drinkers often do better with 5-10-15 minute misting sessions in order to trigger their drinking process. As was mentioned, drying out between sessions minimizes microbial growth. If your room humidity is between 40% and 60% then I would suspect that the humidity level isn't the main source of the eye closures. If your vet will supply it, you could try Gentamicin eye drops. You can use a hypo/needle to more easily squirt a small drop right onto the eyeball from inches away. Although Gentamicin is not a cure-all for all types of eye infections, it offers a reasonable chance to eliminate one potential reason for these eye closures. I'm also not forgetting that you have two chameleons and each is only closing one eye . My experience is that this is more often not an eye infection but related to an environmental stress condition (or some other entirely different illness). When it isn’t an eye infection, think of one eye and then two eyes being closed as sort of the equivalent of a human wincing in pain. Post photos of the entire room showing the enclosures and their locations as well as more photos showing the enclosure interiors. What may be required is to eliminate as many deviations from the "golden reference" Panther setup in order to eliminate potential sources of the problem. Think of it sort of like re-imaging your PC after an infection .

Typical example of Gentamicin (usually prescription): http://www.drsfostersmith.com/produc...fm?pcatid=9721

__________________
See Ya,

Dave Weldon
Panther & Veileds

Book Collection:
"Reptile Medicine and Surgery" 2nd ed. By Mader
"Chameleons - Their Care and Breeding" By Linda J. Davison
"Chameleons - Nature's Hidden Jewels" 2nd Ed. By Petr Necas
"The Panther Chameleon: Color Variation, Natural History, Conservation and Captive Management" By Gary W. Ferguson...
"Thoughts for Food" 3rd Ed. Edited by Ardi Abate
"Understanding Reptile Parasites" By Roger J. Klingenberg D.V.M.

I tried using a reptisun 10.0 with everyone one of my chameleons and it all came out with the same result, EYE problems just like yours.
Try turning off the uvb tubes, I have no idea why its happening to your female having a 5.0 tube maybe its faulty or something.
But my chams would be the same like your male where they would keep there eyes closed for long but would make a bit of effort to open it if it feels the vibrations of you busy in the cage.
How far are the chameleons from the uvb tube?
If they are getting so close that there eye(s) are nearly touching the tube then that could be the problem, but try turning off the uvb for maybe a few days and maybe get new 5.0 tubes if you turn them off for a few days you would probably notice the female starting to open her eyes a bit and the same with the male and after a week or more there eyes will be fine.

My chameleon had an eye infection when i got it. I took it to the vet and they gave me ocufolx ( broad spectrum antibiotic drop)d to administer 3 times a day and it cleared it right up.

Ive had to deal with an eye problem on my Louie, and boy it wasnt fun! I tried everything, but using Vit A. capsules helped him alot! Therefor I think he has a vit A defficiency. This isnt the exact bottle I use(it was linked to me by lbesok, she uses this one though and it worked for her), but Id get something very similar. All you do is poke the capsule with a pin or something sharp, squeeze it out onto a Q-tip, find someway to open your chams mouth and rub it up on the top of his mouth. He/she will hopefully bite down and get the Vit A. Also you can rub the stuff on a cricket or some sort of feeder. Do this every other days for 2-3 days maybe(try not to overdo it). Hopefully this provided something to try.

can fish oil be used as a source of vitamin A for chameleons?